by Stie, J. et al.
Montana State University, Department of Microbiology, 109 Lewis Hall, Bozeman, USA
Montana State University, Department of Microbiology, 109 Lewis Hall, Bozeman, USA
Surface association of hCAP-18 on fMLF-stimulated neutrophils was confirmed by immunofluorescence microscopy and flow cytometry analysis, which also suggested a significant up-regulation of surface hCAP-18 on cytochalasin B-pretreated, fully degranulated neutrophils. hCAP-18 surface association was labile to 10 mM NaOH treatment but resistant to 1 M NaCl and also partitioned into the detergent phase following Triton X-114 solubilization, possibly suggesting a stable association with one or more integral membrane proteins.
J. Leukoc. Biol. 2007; 1:161-72